Method for creating perfusable microvessel systems

Details Method for creating perfusable microvessel systems Method for creating perfusable microvessel systems

C - Chemistry, Metallurgy

12

N

C12N 5/00 (2006.01) C12M 3/02 (2006.01) C12N 5/08 (2006.01)

Patent

CA 2646741

Microvessel networks are produced in vitro from tissue-engineered parent vessels (200, 202) sprouting into a supporting matrix, as for example gels, of extracellular matrix proteins. The microvessel systems are integrated into devices that allow for controlled perfusion with fluids. The vessels may include cells from one cell type, for example, endothelial cells, or from combinations of two or more cell types.

La présente invention concerne des réseaux de microvaisseaux produits in vitro par des vaisseaux parents (200, 202) obtenus par ingénierie tissulaire et produisant des rejetons dans une matrice de support, telle que par exemple des gels de protéines matricielles extracellulaires. Les systèmes de microvaisseaux sont intégrés dans des dispositifs qui permettent une perfusion contrôlée avec des fluides. Les vaisseaux peuvent inclure des cellules issues d'un seul type cellulaire, comme par exemple des cellules endothéliales, ou de combinaisons de deux types de cellules ou plus.

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