Method for creating perfusable microvessel systems

Details Method for creating perfusable microvessel systems Method for creating perfusable microvessel systems

C - Chemistry, Metallurgy

12

N

C12N 5/071 (2010.01) C12M 3/00 (2006.01) C12Q 1/02 (2006.01)

Patent

CA 2700371

A method for creating networks of perfusable microvessels in vitro. Cells including cell types capable of sprouting are seeded 1300 into a channel in a matrix at to activate competency 1304 of the cells for sprouting as microvessels based on the seeding density. The matrix channel is perfused with medium to allow parent vessels to form and for viability 1324. The parent vessels and matrix are incubated and perfused to provide for sprouting of microvessels from parent vessels into the surrounding matrix 1328. The sprouting parent vessels are grown until network forms 1332.

L'invention concerne un procédé permettant de créer des réseaux de microvaisseaux perfusables in vitro. Des cellules comprenant des types de cellules capables de bourgeonner sont ensemencées 1300 dans un canal d'une matrice de façon à activer la capacité 1304 des cellules à bourgeonner sous la forme de microvaisseaux en fonction de la densité d'ensemencement. Le canal de matrice est perfusé avec le milieu pour permettre à des vaisseaux parents de se former et pour assurer la viabilité 1324. Les vaisseaux parents et la matrice sont incubés et perfusés pour assurer le bourgeonnement des microvaisseaux à partir des vaisseaux parents dans la matrice environnante 1328. Les vaisseaux bourgeonnants environnants sont cultivés jusqu'à ce qu'un réseau se forme 1332.

Affiliated with

Also associated with

Rating

Method for creating perfusable microvessel systems does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method for creating perfusable microvessel systems, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for creating perfusable microvessel systems will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFCA-PAI-O-1793495