C - Chemistry – Metallurgy – 12 – M
Patent
C - Chemistry, Metallurgy
12
M
C12M 3/00 (2006.01) C12N 5/071 (2010.01) C12M 3/04 (2006.01) C12N 11/00 (2006.01)
Patent
CA 2700364
A method for creating networks of perfusable microvessels (222) in vitro. A mandrel (916) is drawn through a matrix (1054) to form a channel through the matrix (1010). Cells (1) are injected into the channel (1014). The matrix (1054) is incubated to allow the cells (1) to attach inside the channel (1016). The channel is perfused (1020) to remove unattached cells to create a parent vessel (1022), where the parent vessel (1102) includes a perfusable hollow channel lined with cells (1) in the matrix (1054). The parent vessel (1102) is induced to create sprouts (1106) into the surrounding matrix gel (1054) so as to form a microvessel network (222). The microvessel network (222) is subjected to luminal perfusion through the parent vessel (1102).
La présente invention concerne un procédé de création de réseaux de microvaisseaux perfusables (222) in vitro. Un mandrin (916) est tiré dans une matrice (1054) pour former un canal à travers la matrice (1010). Des cellules (1) sont injectées dans le canal (1014). La matrice (1054) est incubée pour permettre aux cellules (1) de se fixer à l'intérieur du canal (1016). Le canal est perfusé (1020) pour enlever les cellules non fixées afin de créer un vaisseau parent (1022) qui comprend un canal creux perfusable tapissé de cellules (1) dans la matrice (1054). Le vaisseau parent (1102) est incité à créer des germes (1106) dans le gel de matrice environnant (1054) de sorte à former un réseau de microvaisseaux (222). Le réseau de microvaisseaux (222) est soumis à une perfusion luminale à travers le vaisseau parent (1102).
Nortis Inc.
Smart & Biggar
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