C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01) C12N 15/10 (2006.01) C12P 19/34 (2006.01) C07K 14/435 (2006.01)
Patent
CA 2313380
The invention provides methods for evolving a polynucleotide toward acquisition of a desired property. Such methods entail incubating a population of parental polynucleotide variants under conditions to generate annealed polynucleotides comprising heteroduplexes. The heteroduplexes are then exposed to a cellular DNA repair system to convert the heteroduplexes to parental polynucleotide variants or recombined polynucleotide variants. The resulting polynucleotides are then screened or selected for the desired property.
L'invention concerne des procédés servant à faire évoluer un polynucléotide vers l'acquisition d'une propriété souhaitée. Ces procédés consistent en l'incubation d'une population de variantes parentales de polynucléotides dans des conditions capables de générer des polynucléotides renaturés comprenant des hétéroduplex. On expose ensuite ces hétéroduplex à un système de réparation d'ADN cellulaire afin de convertir ces hétéroduplex en variantes parentales de polynucléotides ou en variantes de recombinaison de polynucléotides. On effectue ensuite le criblage des polynucléotides obtenus ou leur sélection afin de rechercher la propriété souhaitée.
Arnold Frances
Shao Zhixin
Volkov Alexander
California Institute Of Technology
Smart & Biggar
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