Method for cultivating mycorrhizal fungi and their fruit bodies

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 1/14 (2006.01) A01G 1/04 (2006.01)

Patent

CA 2031627

ABSTRACT The object of this invention is to propose a novel method for cultivating a mycorrhizal fungus, one of the classes Eubasidiomycetes and Euascomycetes, under artlficial conditions. According to a first aspect of the invention we provide a method for cultivating the mycorrhizal fungi comprising: by means of a porous membrane which has fine pores through which a hypha of mycorrhizal fungus can penetrate and pass, but through which an aqueous solution of nutrients does not permeate substantially under cultivating conditions; separating a main zone for cultivating the mycorrhizal fungus substantially from a zone for supplying the aqueous solution of nutrients with the porous membrane; cultivating the mycorrhizal fungus substantially in the main zone; and supplying the aqueous solution of nutrients to the zone for supplying the aqueous solution; and using the porous membrane as an artificial base material with which the mycorrhizal fungus makes mycorrhizas. The porous membrane is composed of a water-repellent material of which the contact angle with water is more than 90 degrees, and is also composed of a composite consisting of the water-repellent material and a wettable material of which the contact angle with water is not more than 90 degrees. Preferably the shape of porous membrane is a pipe. We also provide a method for preparing a fruit body of mycorrhizal fungus by means of fruit body developing procedure with the hyphae of mycorrhizal fungus cultivated. Some fruit bodies of mycorrhizal fungi prepared are edible.

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