Method for culturing a basidiomycetous fungus in a liquid...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 1/14 (2006.01) A01G 1/04 (2006.01) C12M 1/04 (2006.01) C12M 1/16 (2006.01)

Patent

CA 2370817

Disclosed is an efficient method for culturing an edible basidiomycetous fungus such as Mushroom Agaricus Blazei Murill in a liquid culture medium to give fungus aggregates of several centimeter size. Characteristically, the liquid culture medium is formulated with sucrose as a carbon source in the form of crude cane sugar in combination with a water-insoluble growth- supporting material in the form of a fine powder to serve as the core of the fungus aggregates as selected from crushed sugarcane, sugarcane bagasse, pine trees and wheat bran. Further characteristically, the culturing procedure is carried out under an oxygen-enriched condition by blowing oxygen-enriched air of at least 30 % by volume oxygen into the culture medium under pressurization at 0.12 to 0.5 MPa (absolute) in a specified blowing rate.

L'invention concerne un procédé efficace de culture d'un basidiomycète comestible tel que le champignon Agaricus Blazei Murill dans un milieu de culture liquide en vue d'obtenir des agrégats de champignons de plusieurs centimètres. De manière caractéristique, le milieu de culture liquide est formulé avec du sucrose comme source de carbone, sous la forme de sucre de canne brut combiné à une matière de support de croissance insoluble dans l'eau sous forme de poudre fine qui sert de partie centrale des agrégats de champignons, cette matière étant sélectionnée dans le groupe comprenant du sucre de canne concassé, de la bagasse de sucre de canne, des pins et du son de blé. Comme autre caractéristique, la procédure de culture est mise en oeuvre dans des conditions enrichies en oxygène, par l'introduction d'air enrichi en oxygène selon une proportion d'au moins 30 % en volume d'oxygène dans le milieu de culture à une pression de 0,12 à 0,5 MPa (absolu), selon un débit spécifié.

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