C - Chemistry – Metallurgy – 12 – M
Patent
C - Chemistry, Metallurgy
12
M
C12M 3/00 (2006.01) C12N 5/02 (2006.01) C12N 5/10 (2006.01) A61K 35/12 (2006.01) A61K 48/00 (2006.01) C12N 15/85 (2006.01) C12N 5/08 (2006.01) C12N 5/06 (2006.01)
Patent
CA 2100268
Methods, including culture media conditions and reactors, which provide for ex vivo human stem cell division and stable genetic transformation and/or the optimization of human hematopoietic progenitor cell cultures and/or increasing the metabolism or GM-CSF secretion or IL-6 secretion of human stromal cells are disclosed. The methods rely on culturing human stem cells and/or human hemapoietic progenitor cells and/or human stromal cells in a liquid culture medium which is replaced, preferably perfused, either continuously or periodically, at a rate of 1 ml of medium per ml of culture per about 24 to about 48 hour period, and removing metabolic products and replenishing depleted nutrients while maintaining the culture under physiologically acceptable conditions. Optionally growth factors are added to the culture medium.
Clarke Michael F.
Emerson Stephen G.
Palsson Bernhard O.
Schwartz Richard M.
Ogilvy Renault Llp/s.e.n.c.r.l.,s.r.l.
The Regents Of The University Of Michigan
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