Method for culturing microorganisms having methanol...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 1/16 (2006.01) C12N 9/00 (2006.01) C12N 9/60 (2006.01) C12N 15/81 (2006.01) C12P 21/00 (2006.01)

Patent

CA 2198965

The present invention discloses a method for culturing microorganisms having a methanol metabolic pathway in which an expression unit is introduced that comprises a target gene linked downstream from a promoter that can be induced by methanol; wherein, during the culturing period, and including the period during which methanol is continuously or periodically added, the rate of addition is adjusted to a rate equal to or less than the maximum methanol consumption rate of said microorganisms.

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