C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 1/16 (2006.01) C12N 9/00 (2006.01) C12N 9/60 (2006.01) C12N 15/81 (2006.01) C12P 21/00 (2006.01)
Patent
CA 2198965
The present invention discloses a method for culturing microorganisms having a methanol metabolic pathway in which an expression unit is introduced that comprises a target gene linked downstream from a promoter that can be induced by methanol; wherein, during the culturing period, and including the period during which methanol is continuously or periodically added, the rate of addition is adjusted to a rate equal to or less than the maximum methanol consumption rate of said microorganisms.
Kato Nobuo
Magota Koji
Rogi Tomohiro
Sakai Yasuyoshi
Daiichi Asubio Pharma Co. Ltd.
Ogilvy Renault Llp/s.e.n.c.r.l.,s.r.l.
Suntory Limited
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