C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
195/145, 195/47,
C12N 5/04 (2006.01) C12M 3/00 (2006.01) C12M 3/02 (2006.01)
Patent
CA 2011653
ABSTRACT OF THE DISCLOSURE A method for culturing a tissue of a plant in a culture tank to which a liquid medium having a dissolved oxygen con- centration of 10ppm or more in a flow state of giving sub- stantially no vibration to the tissue or the cell and an apparatus for culturing a tissue of a plant according to the foregoing method. In this apparatus, a means for supplying oxygen or a gas containing oxygen to a liquid medium in an aeration tank and a means for forcedly supplying the ob- tained oxygen-dissolved liquid medium to a culture tank are provided, where the oxygen-dissolved liquid medium is sup- plied to the culture tank in a flow state of giving sub- stantially no vibration to the tissue or the cell in the culture tank, particularly preferably in the form of a uni- form piston flow. This invention is applied to the mass proliferation of a cell and the production of a metabolite such as alkaloid or the like because a tissue or a cell of a plant such as an adventitious root, a callus, etc. are cul- tured efficiently at a high proliferation speed and at a igh density by these method and apparatus.
Deno Hiroshi
Sakai Masao
Yukimune Yukihito
Deno Hiroshi
Ltd. Mitsui Petrochemical Industries
Sakai Masao
Smart & Biggar
Yukimune Yukihito
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