Method for culturing transformed yeast

Details Method for culturing transformed yeast Method for culturing transformed yeast

C - Chemistry, Metallurgy

12

N

195/1.37

C12N 1/16 (2006.01) C07K 14/02 (2006.01) C12N 15/00 (2006.01) C12N 15/81 (2006.01)

Patent

CA 1277264

- 1 - Abstract: The invention relates to an improved method for culturing a yeast transformed by a recombinant plasmid, which is prepared by inserting a hepatitis B virus gene into a plasmid carrying a repressible acid phosphatase promoter of a yeast, by controlling of the promoter and thereby producing the HBs antigen. The method comprises culturing the transformed yeast in a semi-synthetic medium containing yeast extract, an inorganic ammonium salt, an inorganic magnesium salt and either one or both of glucose and saccharose.

476515

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