A - Human Necessities – 01 – N
Patent
A - Human Necessities
01
N
A01N 1/02 (2006.01) A61F 2/06 (2006.01) A61F 2/10 (2006.01) A61F 2/14 (2006.01) A61L 27/38 (2006.01) A61L 27/50 (2006.01) A61L 27/60 (2006.01)
Patent
CA 2404007
A method of preserving a tissue equivalent in a frozen state whereby the survival ratio of frozen cells and the biological activity of thawed cells are elevated and the steps are simplified; and a tissue equivalent preserved in a frozen state which is obtained by this method. Cells suspended in a liquid for frozen preservation are inoculated into a substrate and then frozen before the cells adhere to the substrate.
L'invention concerne un procédé de conservation d'un équivalent au tissu à l'état congelé, permettant, d'une part, d'obtenir un taux de survie de cellules congelées et une activité biologique de cellules décongelées élevés, et d'autre part, de réduire les étapes de fabrication. L'invention concerne également un équivalent au tissu conservé à l'état congelé obtenu par ce procédé. Les cellules en suspension dans un liquide conçu pour la conservation par le froid sont introduites dans un substrat puis congelées avant leur fixation au substrat.
Moriyama Takeshi
Nomura Masayo
Yamamoto Naoka
Menicon Co. Ltd.
Osler Hoskin & Harcourt Llp
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