Method for deblocking of labeled oligonucleotides

Details Method for deblocking of labeled oligonucleotides Method for deblocking of labeled oligonucleotides

C - Chemistry, Metallurgy

07

H

C07H 21/00 (2006.01) C07H 21/02 (2006.01) C07H 21/04 (2006.01)

Patent

CA 2375060

The invention relates to a process for deblocking substantially a blocked, detectably labeled oligonucleotide by contacting the blocked detectably labeled oligonucleotide with an effective amount of a nucleophilic amino compound under conditions that result in substantial deblocking of the oligonucleotide, thereby giving the substantially deblocked oligonucleotide.

L'invention concerne un procédé servant à débloquer sensiblement un oligonucléotide bloqué marqué de manière détectable, par la mise en contact de l'oligonucléotide bloqué marqué de manière détectable avec une quantité efficace d'un composé amino nucléophile dans des conditions entraînant un déblocage sensible de l'oligonucléotide, ce qui permet d'obtenir un oligonucléotide sensiblement débloqué.

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