Method for decellularising foreign material for the...

A - Human Necessities – 61 – L

Patent

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A61L 2/02 (2006.01) A61F 2/06 (2006.01) A61F 2/24 (2006.01) A61L 2/16 (2006.01) A61L 27/36 (2006.01)

Patent

CA 2428880

The invention relates to a method for decellularising allogenic and xenogenic foreign material for the subsequent production of bioprostheses, coated with endogenous body cells, whereby the foreign material is firstly treated in a solution of bile acid and then alcohol, each with an intermediate or subsequent rinsing step, in combination with a mechanical action on the tissue and the cells by the force generated from a flowing treatment medium, at least in the final rinsing step. After said treatment the tissue is completely rid of foreign cell material and viruses and represents an excellent starting material for the coating with cells of the bioprosthesis recipient.

L'invention concerne un procédé de décellularisation d'une matière étrangère allogénique et xénogénique pour la production ultérieure de bioprothèses enduites de cellules naissant naturellement dans le corps. Cette matière étrangère est traitée chimiquement d'abord dans un acide biliaire puis dans de l'alcool, une étape de rinçage intermédiaire et ultérieure étant réalisée, en combinaison avec un effet mécanique produit sur le tissu et les cellules au moyen des forces générées par l'écoulement d'un milieu de traitement, au moins au cours de la dernière étape de rinçage. Après ce traitement, ce tissu, qui est complètement libéré de la matière cellulaire étrangère et des virus, constitue un excellent produit de départ pour l'enduction de la bioprothèse à l'aide de cellules du receveur.

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