Method for detecting a target compound using a nucleic acid...

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/68 (2006.01)

Patent

CA 2250525

This invention discloses novel detection methods for determining the presence of a target compound in a substance using nucleic acid ligands as detection molecules. Specifically, the substance is bound to a solid support matrix, such as those used in blot procedures, and detection of the target molecule is accomplished using the affinity and specificity of nucleic acid ligands to the target molecule. The method utilized herein for identifying and preparing said nucleic acid ligands is called SELEX. The method of the present invention is additionally useful to isolate the target compounds from the various substances.

Cette invention porte sur de nouvelles méthodes de détection permettant de constater la présence d'un composé cible dans une substance en utilisant des ligands d'acide nucléique comme molécules de détection. Plus précisément, la substance est fixée à une matrice de support solide, du genre de celles que l'on emploie dans les techniques de transfert, et la détection de la molécule cible se fait par le biais de la spécificité et de l'affinité des ligands d'acide nucléique à l'endroit de la molécule cible. La méthode utilisée ici pour identifier et produire ces ligands d'acide nucléique se nomme SELEX (évolution systématique de ligands par enrichissement exponentiel). La méthode selon cette invention se révèle, de surcroît, des plus utile s'agissant d'isoler les composés cibles de diverses substances.

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