Method for detecting a target nucleic acid

Details Method for detecting a target nucleic acid Method for detecting a target nucleic acid

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C07H 21/00 (2006.01) C07H 21/04 (2006.01) C12P 19/34 (2006.01)

Patent

CA 2189856

A method for detecting a target nucleic acid, which method includes the steps (i) am- plifying the target using acid to obtain an amplification product using a polymerase, a first primer with or without a segment non- contiguous to a first priming sequence, and a second primer with or without a segment non- contiguous to a second priming sequence in the presence of an oligonucleotide which is inca- pable of acting as a primer for the polymerase, wherein the oligonucleotide has at least 5 con- secutive nucleotides fully complementary to at least 5 consecutive nucleoddes of the first primer, and (ii) detecting the presence of the target nucleic acid by monitoring amplification thereof. In particular, the figure illustrates the method wherein the primer contains a segment noncontiguous to its priming sequence.

Procédé de détection d'un acide nucléique cible consistant (i) à amplifier l'acide nucléique cible pour obtenir un produit d'amplification à l'aide d'une polymérase, une première amorce avec ou sans segment non contigu à une première séquence d'amorçage, et une seconde amorce avec ou sans segment non contigu à une seconde séquence d'amorçage, en présence d'un oligonucléotide incapable d'agir comme amorce de la polymérase. L'oligonucléotide possède au moins 5 nucléotides consécutifs totalement complémentaires d'au moins 5 nucléotides consécutifs de la première amorce; et (ii) à détecter la présence de l'acide nucléique cible par le contrôle de l'amplification. La figure illustre un procédé dans lequel l'amorce contient un segment non contigu à sa séquence d'amorçage.

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