Method for detecting a target nucleic acid in a sample

Details Method for detecting a target nucleic acid in a sample Method for detecting a target nucleic acid in a sample

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2746716

The invention relates to a method for detecting a target nucleic acid in a sample using fluorescent probe pairs which include fluorescent reporter and quencher molecules which may be used in hybridization assays and in nucleic acid amplifi-cation reactions, especially polymerase chain reactions (PCR).

La présente invention concerne une méthode de détection d'un acide nucléique cible dans un échantillon en utilisant des paires de sondes fluorescentes qui incluent des molécules de marqueur fluorescent et de stoppeur de fluorescence, ladite méthode pouvant être employée dans des dosages d'hybridation et dans des réactions d'amplification d'acide nucléique, spécialement les réactions en chaîne par polymérase (PCR).

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