Method for detecting analytes based on evanescent...

Details Method for detecting analytes based on evanescent... Method for detecting analytes based on evanescent...

G - Physics

01

N

G01N 33/543 (2006.01) C12Q 1/68 (2006.01) G01N 33/553 (2006.01)

Patent

CA 2526049

The invention provides methods of detecting one or more specific binding analytes, such as nucleic acids and proteins, in the presence of a neutral or anionic polysaccharide, through light scattering techniques, where a change in light scattering caused by the formation of nanoparticle label complexes within the penetration depth of the evanescent wave of a wave guide signals the presence of the analyte.

L'invention concerne des procédés de détection d'un ou plusieurs analytes de liaison spécifiques, tels des acides nucléiques et des protéines, en présence d'un polysaccharide neutre ou anionique, au moyen de techniques de diffusion de lumière, un changement de la diffusion de lumière étant provoqué par la formation de complexes de marques de nanoparticules dans la pénétration en profondeur de l'onde évanescente de signaux de guide d'ondes en présence de l'analyte.

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