Method for detecting, analyzing, and mapping rna transcripts

Details Method for detecting, analyzing, and mapping rna transcripts Method for detecting, analyzing, and mapping rna transcripts

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C07H 21/02 (2006.01) C07H 21/04 (2006.01) C12P 19/34 (2006.01) C12Q 1/70 (2006.01)

Patent

CA 2330731

A genetic analysis method termed "fine array transcript mapping" or "FAT Mapping" is disclosed, which method is useful for detecting and measuring RNA molecules which have been transcribed from a genome. The method can be applied to explore differential expression of a template genome, and for accurately mapping the 5' ends of transcripts which have been expressed. Further, the presence or absence in any particular biological circumstances of a given transcript and its relative concentration can define gene functions or coding capacities. Thus the method relates to mapping and identifying novel and known gene products and investigating gene functions and regulation.

L'invention concerne un procédé d'analyse génétique baptisé "cartographie de transcrits à réseau fin" qui s'utilise pour détecter et mesurer les molécules d'ARN qui ont été transcrites à partir d'un génome. Le procédé peut être utilisé pour explorer l'expression différentielle d'un génome matrice et pour cartographier les extrémités 5' des transcrits exprimés. En outre, la présence ou l'absence dans des conditions biologiques particulières d'un transcrit donné et sa concentration relative peuvent définir les fonctions d'un gène ou les capacités de codage. Par conséquent, ce procédé a trait à la cartographie et à l'identification des produits géniques nouveaux ou connus et aux recherches en matière de fonctions et de régulation des gènes.

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