Method for detecting and classifying nucleic acid hybridization

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) H01J 49/04 (2006.01)

Patent

CA 2378901

The present invention provides a variety of methods for conducting analyses of nucleic acids using a detection system which is sensitive to the unique dielectric properties of different hybridization complexes and which can distinguish between different hybridization complexes directly without the use of labels. Methods using the system to perform sequence checking, expression analysis, de novo sequencing and a variety of other nucleic acid analyses are provided.

La présente invention concerne des procédés variés permettant d'analyser des acides nucléiques au moyen d'un système de détection sensible aux propriétés diélectriques uniques de différents complexes d'hybridation et qui peut distinguer entre les différents complexes d'hybridation directement sans utiliser de marqueurs. En outre, cette invention concerne des procédés d'utilisation du système pour effectuer la vérification de séquence, l'analyse d'expression, le séquençage de novo et diverses analyses d'acides nucléiques.

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