Method for detecting and isolating nuclear transport protein

Details Method for detecting and isolating nuclear transport protein Method for detecting and isolating nuclear transport protein

C - Chemistry, Metallurgy

12

N

C12N 15/09 (2006.01) C12N 15/10 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2287900

A method for conveniently detecting whether or not a DNA to be tested encodes a peptide with nuclear transportability, which comprises transferring, into an eukaryotic host having in its nucleus a promoter region activated when a transcription factor binds thereto and a reporter gene whose expression is induced by the promoter region, a DNA obtained by fusing a DNA encoding the above-mentioned transcription factor from which the nuclear transportability has been eliminated with the DNA to be tested and then detecting the expression of the reporter gene; and a method for completely and efficiently isolating a DNA encoding a peptide having the nuclear transportability, which comprises isolating the DNA to be tested from the cells showing the expression of the reporter gene.

Procédé servant à détecter efficacement si un A D N à contrôler code un peptide présentant une caractéristique de transport nucléaire, ce qui consiste à transférer, dans un hôte eucaryote dont le noyau comporte une région promoteur activée quand un facteur de transcription se fixe à ladite région et un gène marqueur dont l'expression est induite par ladite région promoteur, un A D N obtenu par fusion d'un A D N codant le facteur de transcription ci-dessus, dont on a éliminé la caractéristique de transport nucléaire, et de l'A D N à contrôler, puis à détecter l'expression du gène marqueur. Procédé servant à isoler totalement et efficacement un A D N codant un peptide possédant la caractéristique de transport nucléaire, ce qui consiste à isoler l'A D N à contrôler depuis les cellules présentant l'expression du gène marqueur.

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