Method for detecting and/or quantifying a known function...

Details Method for detecting and/or quantifying a known function... Method for detecting and/or quantifying a known function...

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2354607

The invention concerns a method for detecting a known function, from nucleic acids present in a sample, characterised in that it comprises the following steps: (a) preparing, from the nucleic acids of the sample, nucleic acid molecules comprising the gene(s) coding for the protein(s) corresponding to said function, and the control elements required for the transcription and the translation of said gene(s); (b) in vitro transcription and translation of the nucleic acid molecule prepared in step (a); (c) detecting and/or measuring the function corresponding to the protein(s) produced in step (b).

La présente invention concerne une méthode de détection d'une fonction connue, à partir des acides nucléiques présents dans un échantillon, caractérisée en ce qu'elle comprend les étapes suivantes (a) la préparation, à partir des acides nucléiques de l'échantillon, de molécules d'acides nucléiques comprenant le ou les gènes codant pour la ou les protéines correspondant à ladite fonction, et les éléments de contrôle nécessaires à la transcription et à la traduction du ou desdits gènes; (b) la transcription et la traduction in vitro des molécules d'acide nucléique préparée(s) à l'étape (a); (c) la détection et/ou la mesure de la fonction correspondant à la ou aux protéines produites à l'étape (b).

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