G - Physics – 01 – N
Patent
G - Physics
01
N
G01N 33/543 (2006.01) B01L 3/00 (2006.01) G01N 33/538 (2006.01)
Patent
CA 2405815
The invention relates to a method for detecting and/or quantifying the binding of first molecules (16) to second molecules (18) affine thereto. The inventive method comprises the following steps: a) providing a container (10), wherein the second molecules (18) are immobilised on a wall, b) contacting the second molecules (18) to a solution containing the first molecules (16), c) incubating the container (10) in such a way that the first molecules (16) bind to the second molecules (18) and build up on the wall and d) detecting the changes in concentration of the first molecules (16) in the solution by means of radiation without removing the solution from the container (10), whereby the solution is contained in said container (10).
L'invention concerne un procédé d'identification et/ou de quantification de la liaison de premières molécules (16) à des secondes molécules (18) ayant une affinité pour les premières, caractérisé en ce qu'il consiste a) à prévoir un récipient (10) dans lequel les secondes molécules (18) sont immobilisées sur une paroi, b) à mettre en contact les secondes molécules (18) avec une solution contenant les premières molécules (16), c) à incuber le récipient (10) de telle façon que les premières molécules (16) se lient aux secondes molécules et s'enrichissent au niveau de la paroi, et d) à détecter par rayonnement, sans retirer la solution du récipient (10), la variation de concentration des premières molécules (16) dans la solution contenue dans ledit récipient (10).
Kosak Hans
Krause Jurgen
Fetherstonhaugh & Co.
November Aktiengesellschaft Gesellschaft Fur Molekulare Medizin
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