Method for detecting and quantitating multiple subcellular...

Details Method for detecting and quantitating multiple subcellular... Method for detecting and quantitating multiple subcellular...

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) G01N 33/53 (2006.01) G01N 33/567 (2006.01)

Patent

CA 2580961

A method for detecting and quantitating multiple and unique fluorescent signals from a cell sample is provided. The method combines immunohistochemistry and a fluorescent-labeled in situ hybridization techniques. The method is useful for identifying specific subcellular components of cells such as chromosomes and proteins.

La présente invention se rapporte à un procédé permettant de détecter et de quantifier de multiples signaux fluorescents uniques issus d'un échantillon cellulaire. Le procédé selon l'invention combine les techniques d'immunohistochimie et d'hybridation in situ à marquage fluorescent. Le procédé selon l'invention est utile pour identifier des composants subcellulaires spécifiques de cellules telles que des chromosomes et des protéines.

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