Method for detecting bacteria and fungi

Details Method for detecting bacteria and fungi Method for detecting bacteria and fungi

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2698476

The present invention relates to methods and means for determining pathogenic fungi in a sample material, e.g. blood. In the method, the bacterial DNA is initially enriched from the total DNA of the sample material, and then the enriched DNA is amplified with specific primer pairs. Detection of the obtained amplicons allows the accurate identification of bacteria and fungi contained in the sample material and of their resistances. The methods and means of the invention allow an early diagnosis of inflammatory diseases, in particular involving non-detected infection (SIRS), and of infectious diseases such as sepsis, spontaneous bacterial peritonitis and endocarditis.

L'invention concerne un procédé et des moyens de détermination de champignons pathogènes dans un matériau échantillon, par exemple, du sang. Dans la mise en oeuvre du procédé, l'ADN bactérienne est tout d'abord enrichie par l'ADN total du matériau échantillon, puis l'ADN enrichie est amplifiée par des paires d'amorces spécifiques. La détection du produit amplifié obtenu permet d'identifier exactement les bactéries et les champignons contenus dans le matériau échantillon et de déterminer leurs résistances. Le procédé et les moyens selon l'invention permettent une détection précoce des affections inflammatoires, en particulier des infections non décelables (SIRS), et des maladies infectieuses, telles que sepsie, péritonite bactérienne spontanée et endocardite.

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