C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01) C12N 15/09 (2006.01)
Patent
CA 2618650
A method of detecting a tumor marker for the diagnosis of large bowel cancer and adenomatous polyposis coli comprising the steps of a) collecting and freezing feces, b) homogenizing the frozen feces in the presence of an RNAase inhibitor and preparing a suspension, c) extracting RNA from the obtained sample from which RNA is extracted, d) obtaining cDNA by reverse transcribing the extracted RNA, e) amplifying the obtained cDNA, and f) detecting the amplified cDNA, characterized in that the tumor marker is one or more tumor markers selected from the group consisting of COX-2, SNAIL and MMP-7 (with the proviso that a case where only COX-2 or MMP-7 is selected is excluded).
L'invention concerne un procédé de détection de marqueur tumoral pour le diagnostic du cancer du gros intestin et du coli polyposis adénomateux comprenant les phases suivantes : a) collecte et congélation des excréments, b) homogénéisation des excréments congelés en présence d'un inhibiteur ARN-ase et préparation d'une suspension, c) extraction d'ARN à partir de l'échantillon obtenu duquel on a extrait l'ARN, d) obtention de cADN par transcription inverse de l'ARN extrait, e) amplification du cADN obtenu, et f) détection du cADN amplifié, caractérisé en ce que le marqueur tumoral est un ou plusieurs marqueurs tumoraux sélectionnés parmi le groupe consistant en COX-2, SNAIL et MMP-7 (à l'exclusion du cas de figure dans lequel seulement COX-2 ou MMP-7 est sélectionné).
Hamamatsu Foundation For Science And Technology Promotion
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