G - Physics – 01 – N
Patent
G - Physics
01
N
167/45
G01N 33/546 (2006.01) C12Q 1/00 (2006.01) C12Q 1/34 (2006.01) C12Q 1/42 (2006.01) G01N 33/543 (2006.01) G01N 33/563 (2006.01) G01N 33/577 (2006.01)
Patent
CA 1254136
ABSTRACT OF THE DISCLOSURE A method is disclosed for determining enzymatic activity in a liquid sample, comprising: 1. Forming an agglutination system comprising: (i) highly refractive particles having a ligand disposed thereon. (ii) a binding partner specific for the ligand and capable of binding to at least two ligands, the binding partner and ligand being present at concentrations which permit agglutination, and (iii) A substrate for the enzyme, the enzyme, substrate, ligand and binding partner being such that a reaction between the enzyme and substrate produces a product which competes with the ligand for the binding partner; 2. contacting the agglutination system with the test sample to produce the product which competes with the ligand for the binding partner; 3. measuring a physical property of the agglutination system, which property is a function of agglutination; and 4. relating the measurement to the amount of enzyme initially present in the test sample.
494310
Ballas Robert A.
Frey William A.
E. I. Du Pont de Nemours And Company
Mccallum Brooks & Co.
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