Method for detecting escherichia coli

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/00 (2006.01) C12Q 1/10 (2006.01) C12Q 1/37 (2006.01)

Patent

CA 2514938

Described herein are methods of detecting an infection and for detecting the presence or absence of microorganisms, for example, wound pathogens in a sample, by contacting a sample with an enzyme produced and/or secreted by the bacteria, and detecting modification or the absence of modification of the substrate, as an indicator of the presence or absence of the enzyme in the sample. The present invention also features a biosensor for detecting the presence or absence of bacteria in a sample.

L'invention concerne des méthodes qui permettent de détecter une infection ou la présence ou l'absence de micro-organismes, tels que des agents pathogènes de blessure, dans un prélèvement, par placement d'un prélèvement au contact d'une enzyme produite et/ou sécrétée par les bactéries; et de détecter une modification ou l'absence de modification du substrat, comme indicateur de la présence ou de l'absence de l'enzyme dans le prélèvement. L'invention concerne en outre un biocapteur pouvant détecter la présence ou l'absence de bactéries dans un prélèvement.

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