C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01) C07H 21/04 (2006.01)
Patent
CA 2223024
A method for detecting Byssochlamys nivea, Neosartorya fischeri and Zygossaccharomyces bailii micro-organisms by amplifying the genomic DNA of the targeted micro-organisms using as a primer a sequence contained on the internal transcript spacers (ITS) of the ribosomal unit, namely, for Byssochlamys nivea, on ITS1 corresponding to SEQ ID 1 and on ITS2 corresponding to SEQ ID 2; for Neosartorya fischeri, on ITS1 corresponding to SEQ ID 3, and on ITS2 corresponding to SEQ ID 4; and for Zygosaccharomyces bailii, on ITS1 corresponding to SEQ ID 5 and on ITS2 corresponding to SEQ ID 6.
La présente invention concerne un procédé permettant la mise en évidence de Byssochlamys nivea, Neosartorya fischeri et Zygosaccharomyces bailii par amplification de l'ADN génomique des microorganismes ciblés en utilisant comme amorce une séquence comprise dans les espaceurs transcrits internes (ITS) de l'unité ribosomique: pour Byssochlamys nivea, sur ITS1 correspondant à SEQ ID 1, sur ITS2 correspondant à SEQ ID 2; pour Neosartorya fischeri, sur ITS1 correspondant à SEQ ID 3, sur ITS2 correspondant à SEQ ID 4; pour Zygosaccharomyces bailii, sur ITS1 correspondant à SEQ ID 5, sur ITS2 correspondant à SEQ ID 6.
Aufrere Robert
Chrzavzez Emmanuelle
Ogilvy Renault Llp/s.e.n.c.r.l.,s.r.l.
Ultra Propre Nutrition Industrie Recherche (u.n.i.r.)
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