Method for detecting human papillomavirus mrna

Details Method for detecting human papillomavirus mrna Method for detecting human papillomavirus mrna

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C12Q 1/70 (2006.01)

Patent

CA 2472026

An in vitro method is provided for screening human female subjects to assess their risk of developing cervical carcinoma which comprises screening the subject for expression of mRNA transcripts from the E6 and optionally the L1 gene of human papillomavirus, wherein subjects positive for expression of L1 and/or E6 mRNA are scored as being at risk of developing cervical carcinoma. Kits for carrying out such methods are also provided.

L'invention concerne une méthode in vitro qui permet de cribler des sujets féminins humains afin d'évaluer leurs risques de développer un carcinome cervical. Cette méthode consiste à cribler le sujet afin de déterminer l'expression de transcripts d'ARN messager de E6 et éventuellement le gène L1 du papillomavirus humain. Les sujets positifs en ce qui concerne l'expression de L1 et/ou de l'ARN messager de E6 sont notés comme présentant un risque de développer un carcinome cervical. L'invention concerne également des trousses permettant de réaliser ces méthodes.

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