G - Physics – 01 – N
Patent
G - Physics
01
N
G01N 33/68 (2006.01) G01N 33/543 (2006.01) G01N 33/569 (2006.01) G01N 33/571 (2006.01) G01N 33/576 (2006.01)
Patent
CA 2329687
The present invention relates to a method for the detection of antibodies and/or antibody isotypes specific for at least two different phases of an infection. The method of the invention is characterized by the simultaneous incubation of a sample from a patient with at least two preferably antigenic compounds. These compounds comprise an epitope that is recognized by an antibody/antibody belonging to a particular isotype wherein said antibody is specifically occurring in one of said phases of said infection. The method of the invention also requires that at least two epitopes are present in the incubation reaction that are specific for different phases of the infection and thus bind antibodies/antibody isotypes specific for said at least two different phases of said infection. The invention also relates to a kit useful for carrying out the method of the invention.
La présente invention concerne une méthode de détection d'anticorps et/ou d'isotypes d'anticorps spécifiques à au moins deux phases différentes d'une infection. La méthode selon l'invention se caractérise par l'incubation simultanée d'un échantillon provenant d'un patient avec deux composés de préférence antigènes. Ces composés comprennent un épitope qui est reconnu par un anticorps/anticorps appartenant à un isotype particulier, cet anticorps intervenant spécifiquement dans une des phases de l'infection. La méthode selon l'invention nécessite également la présence dans la réaction d'incubation d'au moins deux épitopes spécifiques aux différentes phases de l'infection qui lient ainsi des isotypes anticorps/anticorps spécifiques à au moins deux phases différentes de l'infection. L'invention concerne également une trousse s'utilisant pour réaliser la méthode selon l'invention.
Barbero Luca Maria
Barbieri Ugo
Bonelli Fabrizio
Corno Federico
Soleti Antonio
Diasorin International Inc.
Diasorin Srl
Goudreau Gage Dubuc
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