Method for detecting ligands and targets in a mixture

Details Method for detecting ligands and targets in a mixture Method for detecting ligands and targets in a mixture

G - Physics

01

N

G01N 33/543 (2006.01) C07K 1/04 (2006.01) C07K 1/14 (2006.01) C07K 5/087 (2006.01) C07K 5/09 (2006.01) C07K 5/097 (2006.01) C07K 5/103 (2006.01) C07K 7/06 (2006.01) G01N 33/545 (2006.01) G01N 33/548 (2006.01) G01N 33/552 (2006.01) G01N 33/554 (2006.01) G01N 33/569 (2006.01)

Patent

CA 2482529

The invention provides a method of charaterizing a target that binds to a ligand. The method comprises providing ligands, optionally attached to a support, and contacting the ligands with targets to allow at least one target to bind to at least one ligand. The method further comprises immobilizing the resulting complexes in a first matrix, such that each complex has a different position within the first matrix, and transferring the target of the complex to a second matrix. The position of the target within the second matrix corresponds to the position of the ligand-support complex within the first matrix. The target on the second matrix is then detected.

L'invention concerne un procédé permettant de caractériser une cible qui se lie à un ligand, qui consiste à produire des ligands, éventuellement fixés à un support, et à les mettre en contact avec des cibles afin qu'au moins une cible puisse se lier à au moins un ligand. Le procédé consiste en outre à immobiliser dans une première matrice les complexes résultants, de façon que chaque complexe ait une position différente dans la première matrice, et à transférer la cible du complexe vers la seconde matrice. La position de la cible correspond à celle du complexe ligand-support dans la première matrice. La cible sur la seconde matrice est alors détectée.

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