G - Physics – 01 – N
Patent
G - Physics
01
N
150/9
G01N 21/64 (2006.01) G01N 33/52 (2006.01)
Patent
CA 1200477
ABSTRACT OF THE DISCLOSURE A method for determining a time dependent change in the luminescent polarization of a living cell-dye system having a luminescent probe associated with the living cells, where the living cell-dye system is dispersed in a medium. The method comprises introducing a dye into the medium to associate a luminescent probe with the living cells of the living cell-dye system where luminescent components of the dye, which are unassociated with the living cells, increase in concentration over time in the medium to provide a background luminescence. Polarized light in a distinct orientation having a significant vertical orthogonal component is irradiated onto the living cell-dye system and medium. The polarized light emissions from the luminescent probe associated with the living cells and luminescent components in the medium are analyzed in two distinct orientations representing horizontal and vertical orthogonal components of the polarized light emissions to determine the time dependence of the components of the polarized emissions from the luminescent probe associated with the living cells of the system and the luminescent components of the medium. The steps of generating polarized light and analyzing the polarized light emissions are conducted during a period commencing at times greater than the luminescence lifetime of the living cell-dye system and continuing until the background luminescence from the system medium significantly affects the polarized light emission intensities from the luminescent probe associated with the living cells of the system.
431930
Lyke Richard L.
Ware William R.
Pra International Inc.
Sim & Mcburney
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