Method for detecting microbial pathogens employing a...

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G - Physics

01

N

150/11

G01N 33/48 (2006.01) C12Q 1/24 (2006.01) G01N 33/72 (2006.01) G01N 33/80 (2006.01) G01N 33/86 (2006.01) G01N 33/96 (2006.01)

Patent

CA 1098429

METHOD FOR DETECTING MICROBIAL PATHOGENS EMPLOYING A CUSHIONING AGENT Abstract of the disclosure An improved method and apparatus are described for detecting microbial pathogens within a sample fluid, such as blood, in which a confined sterile zone containing the sample fluid is centrifuged. According to the novel feature, the centrifugation is carried out in the presence of a high density, non-toxic, water immiscible, liquid cushioning agent, such as a fluorinated hydrocarbon. The apparatus includes an enclosed centrifugation receptacle sealably closed with injectable closure means, the interior of the receptacle comprising an evacuated space maintain- ed at a lower than atmospheric pressure, adjacent the dushioning agent which is capable of collecting substantially all of the microbial pathogens which pass out of sus- pension from the sample fluid without loss of microbial pathogens to interstitial spaces present in the receptacle.

290205

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