C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/04 (2006.01) C12Q 1/37 (2006.01) G01N 33/543 (2006.01)
Patent
CA 2648359
A method for detecting a microorganism in a sample containing or suspected of containing said microorganism, said method comprising: i) contacting said sample with a binding agent for said microorganism, wherein the binding agent is immobilised on a support, and allowing the binding agent to bind microorganism to form an immobilised complex; ii) separating the sample from the immobilised complex; iii) contacting the support with a liquid medium and a reagent which removes which eliminates, inactivates or inhibits a contaminant that may interfere with a microorganism detection assay; and iv) detecting microorganisms retained on the support using said microorganism detection assay.
L'invention concerne une méthode pour détecter un micro-organisme dans un échantillon qui contient ou est suspecté de contenir ledit micro-organisme, ladite méthode consistant à : i) mettre ledit échantillon en contact avec un liant pour ledit micro-organisme, ledit liant étant immobilisé sur un support, et permettre au liant de se lier audit micro-organisme pour former un complexe immobilisé ; ii) séparer l'échantillon du complexe immobilisé ; iii) mettre en contact le support avec un milieu liquide et un réactif qui retire, élimine, désactive ou inhibe un contaminant qui peut perturber un test de détection du micro-organisme ; et iv) détecter les micro-organismes retenus sur le support en utilisant ledit test de détection de micro-organisme.
Alaska Food Diagnostics Limited
Bereskin & Parr Llp/s.e.n.c.r.l.,s.r.l.
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