Method for detecting microorganisms

C - Chemistry – Metallurgy – 12 – Q

Patent

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Details

C12Q 1/04 (2006.01) C12Q 1/02 (2006.01) C12Q 1/34 (2006.01) C12Q 1/37 (2006.01) G01N 33/569 (2006.01)

Patent

CA 2474458

Described herein are methods of detecting a wound infection and for detecting the presence or absence of microorganisms, for example, wound pathogens in a sample, by contacting a sample with an enzyme produced and/or secreted by the bacteria, and detecting modification or the absence of modification of the substrate, as an indicator of the presence or absence of the enzyme in the sample. The present invention also features a biosensor for detecting the presence or absence of bacteria in a sample.

L'invention concerne des méthodes de détection de l'infection d'une plaie et de détection de la présence ou de l'absence de micro-organismes, par exemple, des agents pathogènes dans un échantillon, par mise en contact d'un échantillon avec une enzyme produite et/ou sécrétée par les bactéries, et par détection de la modification ou de l'absence de modification du substrat, comme indication de la présence ou de l'absence de l'enzyme dans l'échantillon. La présente invention concerne également un biocapteur de détection de la présence ou de l'absence des bactéries dans un échantillon.

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