Method for detecting negatively supercoiled dna

Details Method for detecting negatively supercoiled dna Method for detecting negatively supercoiled dna

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C12Q 1/04 (2006.01) G01N 21/77 (2006.01) G01N 21/78 (2006.01) G01N 33/483 (2006.01) G01N 33/53 (2006.01) G01N 33/58 (2006.01) G01N 21/64 (2006.01)

Patent

CA 2447762

To detect intracellular negatively supercoiled DNA conveniently and efficiently. A method for detecting negatively supercoiled DNA in cells, characterized by including the steps of incorporating biotinylated psoralen into cells, irradiating the cells with long-wavelength UV rays, causing the cells to react with adivin which has been labeled with a color-developing substance, a fluorescent substance, or a chemiluminescent substance, and measuring developed color, emitted fluorescence, or emitted chemiluminescence of the cells.

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