Method for detecting nucleic acid sequence variations

Details Method for detecting nucleic acid sequence variations Method for detecting nucleic acid sequence variations

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2211666

A method of analyzing nucleic acid-containing samples for sequence variations relative to standard nucleic acid sequences, which method comprises providing a single-stranded standard nucleic acid sequence immobilized on a solid support, hybridizing a nucleic acid strand derived from a sample to the immobilized strand, subjecting the nucleic acid complex formed to (i) mismatch- induced cleavage or (ii) mismatch-terminated extension reactions, and detecting possible cleavage or extension-termination by optical measurement on the solid support.

L'invention se rapporte à un procédé d'analyse d'échantillons contenant de l'acide nucléique visant à identifier des variations de séquences par rapport à des séquences normales d'acide nucléique. Ce procédé consiste à obtenir une séquence d'acide nucléique normale monocaténaire immobilisée sur un support solide, à hybrider un brin d'acide nucléique dérivé d'un échantillon sur le brin immobilisé, à soumettre le complexe d'acide nucléique obtenu à (i) un clivage induit par un mésappariement ou (ii) à des réactions d'allongement terminées par un mésappariement, et à détecter un éventuel clivage ou allongement-terminaison par mesure optique sur le support solide.

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