Method for detecting nucleic acid sequences

Details Method for detecting nucleic acid sequences Method for detecting nucleic acid sequences

C - Chemistry, Metallurgy

12

Q

150/8.5

C12Q 1/68 (2006.01)

Patent

CA 1341495

This invention provides a method for detecting a target nucleic acid molecule which comprises forming a reaction mixture which includes the target nucleic acid molecule and an amount of a complementary single-stranded nucleic acid probe which is greater than the target molecule, under conditions which allow the probe and the target nucleic acid molecule to hybridize to each other and form a double stranded target-probe complex nicking the hybridized probe at least once within a predetermined sequence so as to form at least two probe fragments hybridized to the target nucleic acid molecule, resulting in the probe fragments to become single-standed and allowing the target nucleic acid molecule to become hybridized to another probe; and identifying probe fragments, thereby detecting the target nucleic acid molecule. This invention also provides a method for detecting a target de-oxyribonucleic acid molecule.

597529

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