Method for detecting or enriching a target microorganism...

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/10 (2006.01)

Patent

CA 2586299

Compositions containing bacteriophages and methods of using bacteriophages in microorganism detection assays and microbial growth and plating media are disclosed. The lytic ability of these phages to control the growth of non- target populations provides superior sensitivity and specificity to detection assays and reduces false negative and false positive results. The removal of contaminating bacteria reduces the microbial competition for nutrients in the growth media thereby increasing the efficiency and productivity of the culture. The phage treatment of the sample increases the proportion of target microorganisms in the sample over contaminating bacteria thereby requiring less time for enrichment to obtain a significant signal improving overall signal to noise ratio in assays and providing for higher yield of end product in microbiological production systems.

L'invention concerne des compositions contenant des bactériophages et des procédés d'utilisation de bactériophages lors de dosages de détection de micro-organismes ainsi que la croissance microbienne et des milieux de mise en culture. La capacité lytique de ces phages à combattre la croissance de populations non ciblées fournit une sensibilité et une spécificité supérieures en matière de dosage de détection et réduit les résultats faux négatifs et faux positifs. L'élimination des bactéries contaminantes réduit la concurrence microbienne pour les nutriants dans les milieux de mise en culture, ce qui augmente l'efficacité et la productivité de la culture. Le traitement phagique de l'échantillon augmente la proportion des micro-organismes cibles dans ledit échantillon par rapport aux bactéries contaminantes, et l'enrichissement requiert alors moins de temps pour obtenir un signal significatif améliorant le rapport global signal-bruit dans les dosages, et le rendement du produit final est par conséquent supérieur dans des systèmes de production microbiologiques.

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