C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01)
Patent
CA 2553286
The Present invention relates to detection of pathogenic mycobacteria in clinical specimens such as sputum, cerebrospinal fluid, gastric lavage and tissue biopsies etc., wherein the novel stretch of DNA that lies in the intergenic region between methyl mycolic acid synthase genes mmaA1 and mmaA2 and the flanking region in mmaA1 and mmaA2 genes and is the invention uses a pair of designed oligonucleotide primers that specifically amplifies the target DNA from the clinical specimens.
La présente invention se rapporte à la détection de mycobactéries pathogènes dans les spécimens cliniques tels que les crachats, le liquide céphalo-rachidien, le liquide issu d'un lavage gastrique, les biopsies tissulaires, etc., le nouveau domaine d'ADN se trouvant dans la région intergénique comprise entre les gènes mmaA1 et mmaA2 de la synthase de l'acide méthyle-mycolique et la région flanquante dans les gènes mmaA1 et mmaA2 et constituant l'invention utilisant une paire d'amorces oligonucléotidiques conçues spécifiquement pour amplifier l'ADN cible des spécimens cliniques.
Hari Das Rakha
Kumar Ajay
Singh Meghpati
Council Of Scientific And Industrial Research
Ridout & Maybee Llp
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