Method for detecting peptides comprising a cross-beta structure

Details Method for detecting peptides comprising a cross-beta structure Method for detecting peptides comprising a cross-beta structure

G - Physics

01

N

G01N 33/68 (2006.01) C07K 1/14 (2006.01) C07K 14/765 (2006.01)

Patent

CA 2614941

The invention relates to the field of aqueous solutions comprising a protein. More specifically, the invention relates to the detection and/or removal of conformationally altered proteins and/or peptides comprising a cross-~ structure from an aqueous solution comprising a protein. The invention provides methods for detecting and/or removing proteins and/or peptides comprising a cross-~ structure from an aqueous solution comprising a protein, said method comprising contacting said aqueous solution comprising a protein with at least one cross-~ structure-binding compound resulting in a bound protein or peptide with cross-~ structure. The invention further provides a aqueous solution comprising a protein obtainable by a method of the invention, and a kit for carrying out the methods of the invention.

L'invention concerne des solutions aqueuses comprenant une protéine, et plus spécifiquement, la détection et/ou l'élimination de protéines dont la conformation a été modifiée et/ou de peptides, comprenant une structure croisée .beta., d'une solution aqueuse comprenant une protéine. L'invention concerne également des procédés de détection et/ou d'élimination de protéines et/ou de peptides, comprenant une structure croisée .beta., d'une solution aqueuse comprenant une protéine, ce procédé consistant à mettre en contact la solution aqueuse comprenant un protéine avec au moins un composé de liaison à structure croisée .beta. résultant d'une protéine liée ou d'un peptide à structure croisée .beta.. L'invention concerne également une solution aqueuse comprenant une protéine pouvant être obtenue par le procédé de cette invention, et un kit pour réaliser les procédés de l'invention.

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