C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01)
Patent
CA 2726571
A method and kit for detection and identification in a test sample of hMPV, are described, as is a set of sequences and their use as amplification primers for hMPV. hMPV amplification according to the present invention is compatible with detection of other viruses that cause respiratory tract infections, as well as with genotyping of hMPV present in the sample. After subjecting the test sample to amplification with primers specific for each virus, single-stranded DNA is obtained from the amplification products, and hybridised to virus-specific probes.
Linvention concerne un procédé et un kit pour la détection et lidentification de hMPV dans un échantillon de test, ainsi quun ensemble de séquences et leur utilisation en tant quamorces d'amplification pour le hMPV. Lamplification du hMPV selon la présente invention est compatible avec la détection dautres virus responsables dinfections du tractus respiratoire, ainsi quavec le génotypage du hMPV présent dans léchantillon. Après avoir soumis léchantillon de test à une amplification à laide damorces spécifiques à chaque virus, un ADN simple brin est obtenu à partir des produits de lamplification, et hybridé avec les sondes spécifiques du virus.
Aleman Ainel
Moscoso Del Prado Juan
Villahermosa Jaen Maria L.
Genomica S.a.u.
Marks & Clerk
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