C - Chemistry – Metallurgy – 07 – K
Patent
C - Chemistry, Metallurgy
07
K
C07K 16/12 (2006.01) G01N 1/28 (2006.01) G01N 33/554 (2006.01) G01N 33/569 (2006.01) G01N 33/58 (2006.01)
Patent
CA 2427693
Antigen-specific antibodies of enhanced sensitivity and specificity to target bacterial antigens selected from among lipo-polycarbohydrate antigens, antigens comprising lipoteichoic acid or teichoic acid or a derivative of either and capsular carbohydrate antigens may be obtained by (1) extraction from a culture of known gram-negative or gram-positive bacteria of the target antigen containing not more than 10% protein by weight, (2) coupling the conjugate to an affinity column, passing raw polyclonal antibodies to the target antigen or an IgG cut thereof over the affinity column and eluting from the column purified antibodies specific to the target antigen. A rapid immunochromatographic test is described in which the thus purified antibodies are used to detect in a mammalian bodily fluid the target antigen or the bacterium from which it was extracted. Use of the the purified antibodies in any known test for the purpose of detecting the presence or absence of target antigen (or target bacterium) is further contemplated.
Fent Mary Kathleen
Koulchin Vladimir Andrei
Molokova Elena Valentin
Moore Norman James
Binax Inc.
Sim & Mcburney
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