Method for detection of alkylated cytosine in dna

Details Method for detection of alkylated cytosine in dna Method for detection of alkylated cytosine in dna

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2530506

There is described a method for detecting alkylated cytosine in double stranded DNA employing one or more enzymes that differentially modify alkylated cytosine and cytosine. At least one region of the DNA is converted to single stranded DNA and the enzyme is reacted with a target region in the single stranded DNA. The presence or level of alkylated cytosine in the target region is detected by determining the level of enzymatic modification of the target region by the enzyme.

Cette invention se rapporte à un procédé servant à détecter la cytosine alkylée dans un ADN bicaténaire, en utilisant une ou plusieurs enzymes qui modifient de façon différentielle la cytosine alkylée et la cytosine. Au moins une zone de l'ADN est convertie en ADN monocaténaire et l'enzyme est amenée à réagir avec une zone cible de l'ADN monocaténaire. On détermine la présence ou le niveau de cytosine alkylée dans la zone cible en déterminant le niveau de modification enzymatique de la zone cible par l'enzyme.

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