Method for detection of gram-negative bacterial...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/56 (2006.01) G01N 33/569 (2006.01) G01N 33/577 (2006.01) G01N 33/579 (2006.01)

Patent

CA 2084083

2084083 9201228 PCTABS00010 The invention relates to a method of detecting gram-negative bacterial endotoxin using antibody capture combined with amoebocyte lysate chromogenic detection. The method is highly sensitive and rapid and may be used for detection of specific endotoxin. In a particular application, picogram levels of Haemophilus influenzae) type b endotoxin are detected in plasma taken from previously infected mammals. In another particular application, the method is applied to the detection and diagnosis of disease, through the detection of endotoxin from disease-causing organisms. A specific example is the diagnosis of chancroid through the detection of endotoxin from H. ducreyi.

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