Method for detection of herpesvirus in a test sample

Details Method for detection of herpesvirus in a test sample Method for detection of herpesvirus in a test sample

C - Chemistry, Metallurgy

12

Q

C12Q 1/70 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2720421

A method and kit for detection and identification of Herpesvirus in a test sample, in particular of HSV-1, HSV-2 and/or HHV-6, are described. The test sample is subjected to amplification with particularly advantageous combinations of amplification primers. Amplification may be followed by hybridisation to virus-specific probes. A method and kit further allowing efficient detection of VZV, CMV, EBV, Enterovirus, HHV-7 and HHV- 8 are further described.

L'invention porte sur un procédé et un coffret pour la détection et l'identification d'un herpès virus, en particulier de VHS-1, VHS-2 et/ou VHH-6, dans un échantillon de test. L'échantillon de test est soumis à une amplification avec des combinaisons particulièrement avantageuses d'amorces d'amplification. Une amplification peut être suivie par une hybridation avec des sondes spécifiques du virus. L'invention porte en outre sur un procédé et un coffret permettant en outre une détection efficace de VZV, CMV, EBV, dun entérovirus, de VHH-7 et de VHH-8.

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