Method for detection of methylthioadenosine phosphorylase...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/54 (2006.01) C07K 16/40 (2006.01) C12N 9/10 (2006.01) C12Q 1/25 (2006.01) C12Q 1/68 (2006.01) G01N 33/50 (2006.01) G01N 33/573 (2006.01)

Patent

CA 2179908

A method for the detecting whether methyladenosing phosphatase (MTAse) is present in a cell sample in either a catalytically active or catalytically inactive form. In one respect, the method comprises adding oligonucleotide probes to the sample, which probes are capable of specifically hybridizing to any MTAse encoding nucleic acid in the sample under conditions favoring that hybridization. Absence of MTAse in a sample is considered to be indicative of malignancy. Polynucleotides encoding MTAse, MTAse peptides and antibodies to MTAse, as well as kits for performing the methods of the invention, are provided.

Procédé permettant de détecter si la méthyladénosine-phosphatase (MTAse) est présente dans un échantillon cellulaire sous forme catalytiquement active ou sous forme catalytiquement inactive. Dans un aspect, le procédé consiste à ajouter à l'échantillon des sondes oligonucléotidiques suceptibles d'hybridation spécifique avec un quelconque acide nucléique de codage de MTAse dans l'échantillon dans des conditions favorisant cette hybridation. L'absence de MTAse dans un échantillon est considérée comme un signe de malignité. Sont également décrits des polynucléotides codant pour des MTAse, des peptides de MTAse et des anticorps de MTAse, ainsi que des trousses pour mettre en oeuvre les procédés de l'invention.

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