C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/09 (2006.01) C12Q 1/04 (2006.01) C12Q 1/25 (2006.01) C12Q 1/68 (2006.01)
Patent
CA 2615089
A living microorganism in a test sample can be detected according to the following steps: a) treating the test sample with a topoisomerase inhibitor and/or a DNA gyrase inhibitor; b) extracting DNA from the test sample and amplifying the target region in the DNA by PCR; and c) analyzing the amplification product.
Selon la présente invention, on peut détecter un microorganisme vivant dans un échantillon d'essai conformément aux étapes suivantes qui consistent à : a) traiter l'échantillon d'essai avec un inhibiteur de la topoisomérase et/ou un inhibiteur de l'ADN gyrase ; b) extraire l'ADN de l'échantillon d'essai et amplifier la région cible dans l'ADN par une amplification en chaîne par polymérase (ACP) ; et c) analyser le produit de l'amplification.
Soejima Takashi
Yoshida Shinichi
Morinaga Milk Industry Co. Ltd.
Norton Rose Or S.e.n.c.r.l. S.r.l./llp
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