G - Physics – 01 – N
Patent
G - Physics
01
N
G01N 33/574 (2006.01) G01N 33/573 (2006.01)
Patent
CA 2353267
A method for detecting prostate specific membrane antigen (PSMA) in serum by immunoassay including the steps of capturing PSMA from serum using a PSMA antibody and detecting the captured PSMA with an anti-.alpha.1-antichymotrypsin antibody. In a preferred embodiment, PSMA is captured using a PSMA antibody by diluting the PSMA antibody, coating a surface such as the surface of wells in a microtiter plate, with the resulting solution, blocking non-specific sites on the surface with albumin, diluting the serum with a blocking buffer and applying a diluted sample to the surface to capture PSMA. A preferred embodiment for detecting captured PSMA, includes the steps of removing non- captured material by washing the surface, applying anti-.alpha.1antichymotrypsin antibody diluted in a blocking solution such as albumin solution and detecting bound PSMA using peroxidase labeled anti-rabbit IgG and OPD substrate.
Chadha Kailash C.
Kawinski Elzbieta
Gowling Lafleur Henderson Llp
Health Research Inc.
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