Method for detection of target nucleic acids using pcr

Details Method for detection of target nucleic acids using pcr Method for detection of target nucleic acids using pcr

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C12P 19/34 (2006.01) G01N 21/64 (2006.01)

Patent

CA 2321185

A method for detecting the presence of a target nucleic acid sequence in a sample, said method comprising (a) subjecting said sample to an amplification reaction using a set of nucleotides, at least one of which is fluorescently labelled, (b) contacting amplification product with a probe under conditions in which the probe will hybridise to said target sequence, said probe comprising a reactive molecule which is able to absorb fluorescence from or donate fluorescent energy to said fluorescent labelled nucleotide and (c) monitoring fluorescence of said sample. The method can be used to quantitate the amount of target nucleic acid in the sample as well as to determine sequence characteristics. Kits for effecting the method are also claimed.

L'invention concerne un procédé permettant de détecter la présence d'une séquence d'acide nucléique cible dans un échantillon. Ce procédé consiste (a) à soumettre ledit échantillon à une réaction d'amplification à l'aide d'un ensemble de nucléotides, l'un d'entre eux au moins étant marqué par fluorescence; (b) à mettre en contact un produit d'amplification avec une sonde dans des conditions telles que ladite sonde s'hybride avec ladite séquence cible, la sonde comprenant une molécule réactive capable d'absorber la fluorescence d'un nucléotide marqué fluorescent ou d'émettre de l'énergie fluorescente vers ledit nucléotide; et (c) à surveiller la fluorescence de l'échantillon. Ce procédé peut être utilisé pour quantifier l'acide nucléique cible dans l'échantillon et déterminer des caractéristiques de séquence. L'invention concerne également des kits permettant de mettre ce procédé en oeuvre.

Affiliated with

Also associated with

Rating

Method for detection of target nucleic acids using pcr does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method for detection of target nucleic acids using pcr, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for detection of target nucleic acids using pcr will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFCA-PAI-O-1440312